NAD+ Reconstitution Calculator
Compute exact NAD+ reconstitution volume and dose units.
Open the calculator
Pre-filled with NAD+ defaults — adjust vial size, water volume, and dose to your protocol.
About the NAD+ Reconstitution Calculator
NAD+ research vials are typically supplied in 100 mg, 500 mg, or 1000 mg of lyophilized powder. Because per-dose amounts range widely — from 50 mg to several hundred mg — choosing a reconstitution volume that keeps the dose within a single syringe draw is important.
Enter your vial strength, bacteriostatic water volume, and target dose to get the mg/mL concentration, the mL to draw, and the equivalent insulin units. A 500 mg vial reconstituted with 5 mL of bacteriostatic water yields 100 mg/mL — a 50 mg dose is then 0.5 mL (50 units on a U-100 syringe).
Quick reference
- Typical vial sizes
- 100 mg · 500 mg · 1000 mg
- Typical research dose range
- 50–300 mg per research dose
How to prepare NAD+
- Draw the intended volume of bacteriostatic water into a sterile syringe.
- Slowly inject the water down the inside wall of the NAD+ vial — never directly onto the lyophilized powder.
- Gently swirl (do not shake) until the powder is fully dissolved and the solution is clear.
- Store the reconstituted vial refrigerated at 2–8°C and use within the peptide's stability window.
- Use the concentration and dose volume calculated above to draw each research dose into an insulin syringe.
Frequently asked questions
How do I reconstitute NAD+?
Add bacteriostatic water to the NAD+ vial using the volume the calculator recommends for your desired concentration. Slowly inject the water down the side of the vial, swirl gently, and store the vial refrigerated.
What bacteriostatic water volume should I use for NAD+?
Common choices are 1 mL, 2 mL, or 3 mL of bacteriostatic water. The right volume depends on your vial strength and target dose — use the calculator above to get a concentration that lets you draw your dose in whole insulin units.
What is a typical research dose range for NAD+?
Reported research dose ranges for NAD+ are 50–300 mg per research dose. Always verify against current literature — this tool is for research reference only, not medical advice.
How long is NAD+ stable after reconstitution?
Once reconstituted with bacteriostatic water and stored refrigerated (2–8°C), most peptides remain stable for roughly 28–30 days. Check the specific stability data for NAD+ for your batch.
Can I use this NAD+ calculator with any insulin syringe?
Yes. The calculator supports U-100 syringes in 30, 50, and 100-unit capacities and shows both the mL dose volume and the equivalent insulin units for whichever syringe you choose.
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