TB-500 Reconstitution Calculator
Dial in your TB-500 concentration and dose volume in seconds.
Open the calculator
Pre-filled with TB-500 defaults — adjust vial size, water volume, and dose to your protocol.
About the TB-500 Reconstitution Calculator
TB-500, a synthetic fragment related to Thymosin Beta-4, is typically supplied as a 2 mg, 5 mg, or 10 mg lyophilized vial and reconstituted with bacteriostatic water. Because research protocols usually call for weekly or twice-weekly loading doses of 2–5 mg followed by lower maintenance doses, choosing the right reconstitution volume matters — you want a concentration that draws in a reasonable number of insulin units per dose without wasting vial volume.
Enter your vial strength, the bacteriostatic water you'll add, and your desired dose. The calculator returns the mg/mL concentration, the mL to draw, and the equivalent insulin units, and can also suggest an optimal reconstitution volume that lands each dose on a whole-unit mark.
A common example: a 5 mg vial reconstituted with 2 mL of bacteriostatic water gives 2.5 mg/mL. A 2.5 mg loading dose is then 1 mL (100 units on a U-100 syringe), and a 1 mg maintenance dose is 0.4 mL (40 units).
Quick reference
- Typical vial sizes
- 2 mg · 5 mg · 10 mg
- Typical research dose range
- 2–5 mg loading, then 1–2 mg weekly maintenance
How to prepare TB-500
- Draw the intended volume of bacteriostatic water into a sterile syringe.
- Slowly inject the water down the inside wall of the TB-500 vial — never directly onto the lyophilized powder.
- Gently swirl (do not shake) until the powder is fully dissolved and the solution is clear.
- Store the reconstituted vial refrigerated at 2–8°C and use within the peptide's stability window.
- Use the concentration and dose volume calculated above to draw each research dose into an insulin syringe.
Frequently asked questions
How do I reconstitute TB-500?
Add bacteriostatic water to the TB-500 vial using the volume the calculator recommends for your desired concentration. Slowly inject the water down the side of the vial, swirl gently, and store the vial refrigerated.
What bacteriostatic water volume should I use for TB-500?
Common choices are 1 mL, 2 mL, or 3 mL of bacteriostatic water. The right volume depends on your vial strength and target dose — use the calculator above to get a concentration that lets you draw your dose in whole insulin units.
What is a typical research dose range for TB-500?
Reported research dose ranges for TB-500 are 2–5 mg loading doses followed by 1–2 mg weekly. Always verify against current literature — this tool is for research reference only, not medical advice.
How long is TB-500 stable after reconstitution?
Once reconstituted with bacteriostatic water and stored refrigerated (2–8°C), most peptides remain stable for roughly 28–30 days. Check the specific stability data for TB-500 for your batch.
Can I use this TB-500 calculator with any insulin syringe?
Yes. The calculator supports U-100 syringes in 30, 50, and 100-unit capacities and shows both the mL dose volume and the equivalent insulin units for whichever syringe you choose.
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