TB-500 Reconstitution Calculator

TB-500, a synthetic fragment related to Thymosin Beta-4, is typically supplied as a 2 mg, 5 mg, or 10 mg lyophilized vial and reconstituted with bacteriostatic water. Because research protocols usually call for weekly or twice-weekly loading doses of 2–5 mg followed by lower maintenance doses, choosing the right reconstitution volume matters — you want a concentration that draws in a reasonable number of insulin units per dose without wasting vial volume.

Enter your vial strength, the bacteriostatic water you'll add, and your desired dose. The calculator returns the mg/mL concentration, the mL to draw, and the equivalent insulin units, and can also suggest an optimal reconstitution volume that lands each dose on a whole-unit mark.

A common example: a 5 mg vial reconstituted with 2 mL of bacteriostatic water gives 2.5 mg/mL. A 2.5 mg loading dose is then 1 mL (100 units on a U-100 syringe), and a 1 mg maintenance dose is 0.4 mL (40 units).