PepCalcs

    Peptide Nasal Spray Calculator

    Convert a research vial into an accurate nasal spray with a known mcg-per-spray dose.

    Open the calculator

    Adjust each field to match your vial and target dose.

    About the Peptide Nasal Spray Calculator

    Peptides commonly delivered as nasal sprays — Selank, Semax, PT-141, Kisspeptin-10, Epithalon — need a two-step calculation: first the reconstituted vial concentration, then the volume drawn into a spray bottle and the mcg-per-spray delivered by that bottle.

    This calculator does both. Enter the peptide mg, the mL you extracted into the spray bottle, the bottle's total volume, and either the total number of sprays the bottle delivers or your target dose per spray. You'll get the exact mcg (or mg) per spray, the saline volume to top up with, and the total sprays available.

    Typical spray bottles deliver 0.1 mL per pump; a 30 mL bottle gives roughly 300 sprays. If your vial was 10 mg and you drew 2 mL into a 30 mL bottle (topped up with 28 mL of sterile saline), the whole bottle contains 10 mg — divided over 300 sprays, that's about 33 mcg per spray.

    Quick reference

    Typical research dose range
    Depends on peptide — often 100–500 mcg per spray

    How to prepare this peptide

    1. Reconstitute the peptide vial with bacteriostatic water and confirm mg/mL concentration.
    2. Draw the target extraction volume of reconstituted peptide into an empty nasal spray bottle.
    3. Top the bottle up to its total volume with sterile saline.
    4. Prime the pump and confirm the spray count matches the calculator's estimate.
    5. Use the mcg-per-spray value from the calculator to plan doses.

    Frequently asked questions

    How many sprays does a typical nasal spray bottle deliver?

    Most 30 mL nasal spray pumps deliver ~0.1 mL per spray, giving roughly 300 sprays. Check your bottle's spec — some deliver 0.05 mL (600 sprays) or 0.14 mL (~215 sprays).

    Do I need to add saline?

    Saline dilutes the reconstituted peptide up to your bottle's total volume, giving you a predictable mcg-per-spray dose. Sterile saline (0.9%) is the standard choice.

    Can I use bacteriostatic water instead of saline?

    For very short-term use bacteriostatic water works, but sterile saline is closer to intranasal-physiological pH and osmolarity for regular research spraying.

    Which peptides are commonly used as nasal sprays?

    Selank, Semax, PT-141, Kisspeptin-10, Oxytocin, and Epithalon are the most common — anything with reasonable intranasal bioavailability where a small mcg dose is desired.

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